Cloning and expression of cry2Aa from native Bacillus thuringiensis strain SY49-1 and its insecticidal activity against Culex pipiens (Diptera: Culicidae)

YILMAZ S., Azizoglu U., AYVAZ A., TEMİZGÜL R., Atciyurt Z. B., Karaborklu S.

MICROBIAL PATHOGENESIS, vol.105, pp.81-85, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 105
  • Publication Date: 2017
  • Doi Number: 10.1016/j.micpath.2017.02.016
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.81-85
  • Keywords: Bacillus thuringiensis, Cloning, Bt SY49-1, cry2Aa18, Larvicidal activity, Culex pipiens, SUBSP KURSTAKI, CRYSTAL PROTEINS, PEST INSECTS, GENE, SEQUENCE, LEPIDOPTERA, TOXICITY, BINDING, LARVAE, TOXIN
  • Kayseri University Affiliated: No


Bacillus thuringiensis (Berliner) (Bt) is well known for having toxicity against pest insects because of their ability to form endospores and broad-range activity of their parasporal inclusions. In this study, a new member of cry2A gene from previously characterized native B. thuringiensis SY49-1 strain was cloned, expressed and used for its activity against Culex pipiens (Diptera: Culicidae) larvae. The sequence analysis of the cloned cry2A gene revealed that it encodes a polypeptide of 633 as residues with 99% identity to Cry2Aa protein with expected molecular weight of 70.7 kDa. Bacillus thuringiensis delta-endotoxin nomenclature committee designed our sequence as Cry2Aa18 being a new member of Bt toxins. Bioassays against last instar larvae of C pipiens indicated that Cry2Aa18 has considerable toxicity with LC50 of 630 jig ml(-1). In order to prevent the spread of infectious diseases mediated by C. pipiens, this newly characterized cry2Aa18 gene could constitute as an important biological control tool for controlling mosquito larvae living in freshwater systems and can be used as a good alternative for minimizing the use of chemicals. (C) 2017 Elsevier Ltd. All rights reserved.