Universities are not only institutions that carry out education and research activities, but also workplaces. Therefore, they have obligations in terms of Occupational Health and Safety (OHS) legislation. There are differences in the implementation of the OHS Law No. 6331 in universities and there are difficulties in implementation. The OHS Practice Guide in Public Universities has been published to guide universities due to the different status of their employees, the different organizational structure, and the fact that their units are located in different campuses. It is expected that the implementation guides published for public institutions and private enterprises will be in compliance with the legislation. In this study, the relationship between the OHS Practice Guide in Public Universities and the legal legislation was examined by content analysis method. A coding system consisting of 2 main codes and 6 subcodes was used for analysis. “Expression with too much content according to the legal regulations” is the expression with the most coding (f=40). While no outdated expressions were found according to the legal legislation, 18 expressions were coded as "expressions containing legal gaps". Statements containing legal spaces; It has been observed that the OHS boards and the management are clustered under the headings of OHS commitment. In the study, the contributions of the guide to the universities were evaluated; Items that are out of the guideline or that do not have a complete answer in the guideline were opened for discussion.