The purpose of this study is to investigate the effectiveness of the application of colostrum in aflatoxin toxication. For this aim, a total of 48, 3-4 month old Balb C female mice were used, and 4 groups were established. The animals of group 1 were the control group used. Groups 2, 3 and 4 were given 500 mg/kg bw/day bovine colostrum, 750 mu g/kg bw/day aflatoxin (approximately 77% AFB(1), 15% AFB(2), 5% AFG(1) and 3% AFG(2)) and 500 mg/kg bw/day bovine colostrum plus 750 mu g/kg bw/day aflatoxin, respectively, for 21 days by gavage. The MDA levels and the activities of CAT, SOD and GSH-Px were measured in the blood. Furthermore, levels/activities of the glucose, triglyceride, cholesterol, total protein, albumin, BUN, bilirubin, AST and ALP were performed in serum. These results showed that AF caused negative changes in all the oxidative stress parameters and in some biochemical parameters (triglyceride, total protein, AST and ALP), and applications of colostrum alleviated AF toxicity. It was concluded that colostrum is one of the compounds that can be used as a supportive agent in case of aflatoxin intoxication.