Cytotoxic Effects of Methanolic Extracts from Different Cladonia Species on Human Lung Carcinoma (A549) Cell Line

Kocakaya Z., Şeker Karatoprak G., Kocakaya M.

International Ecology 2018 Symposium, Kastamonu, Turkey, 19 - 23 June 2018, pp.1011

  • Publication Type: Conference Paper / Summary Text
  • City: Kastamonu
  • Country: Turkey
  • Page Numbers: pp.1011
  • Kayseri University Affiliated: No


Introduction: Lichens produce a large number of secondary metabolites of great interest due to antiviral, anti-microbacterial, antitumor, anti-allergic, inhibitory activities of plant growth and the like. It has been suggested that the extracts of Cladonia species, including Cladoniaceae family, were used for different medicines in the folk medicine. Lung cancer is the most common cancer. Therefore, in this study we aimed to investigate the effects of the extracts of Cladonia pyxidata (L.) Hoffm. and Cladonia coniocraea (L.) on human lung carcinoma (A 549) with in vitro cytotoxicity assay.

Material and Methods: Within the scope of this study, 70% methanol extracts of Cladonia species, were prepared. Cytotoxic effects on A 549 cell line were studied with MTT assay.

Results: It has been elucidated that Cladonia coniocraea extract was found to be most cytotoxic extract with 314.678 μg/mL IC50 value on A 549 cell line. Cladonia pyxidata extract showed 13% inhibition effect on the cell viability even at 2mg/mL concentration.

Discussion: Among the two Cladonia species studied, C. coniocraea was found promising on A549 cell line. The inhibitory effect of C. coniocraea extract on lung cancer cell line at different concentrations for 48 and 72 h will be examined in our subsequent studies.