COLLECTION OF, AND SEROLOGICAL AND MOLECULAR VIRAL DISEASE DETECTION IN LOCAL GARLIC (ALLIUM SATIVUM L.) ACCESSIONS COLLECTED FROM NORTHERN CYPRUS


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Yılmaz N. , Fidan H.

Fresenius Environmental Bulletin, vol.30, no.1, pp.569-579, 2021 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 30 Issue: 1
  • Publication Date: 2021
  • Title of Journal : Fresenius Environmental Bulletin
  • Page Numbers: pp.569-579

Abstract

This study was conducted by collecting sam-ples of garlic (Allium sativum L.) and studying its morphological characteristics to determine which vi-ral diseases are causing an economic loss of yield in cultivation areas in Northern Cyprus. Considering their morphological characteristics, 87 garlic geno-types were collected from 6 different locations in Northern Cyprus, 83 of which were infected with one or more viruses. The isolates obtained were tested against Garlic common latent virus (Gar-CLV), Leek yellow strip virus (LYSV), Shallot la-tent virus (SLV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV), and Onion yellow dwarf virus (OYDV). For diagnoses, both double an-tibody sandwich enzyme-linked immunosorbent as-say and reverse transcription polymerase chain reac-tion techniques were used. Eighty-seven garlic-leaf samples and 87 garlic clove samples were tested. The results from these samples were similar. Among the 87 plants tested, 38 (44%) were positive for Gar-CLV, 25 (29%) for LYSV, 15 (17%) for SLV, and 5 (5.7%) for OYDV. GarCLV was found to be the most common virus in the isolates.