Cytotoxic Effects of Methanolic Extracts from Different Cladonia Species on Human Lung Carcinoma (A549) Cell Line

Kocakaya M., Şeker Karatoprak G., Kocakaya Z.

The 4rd International Symposium on EuroAsian Biodiversity, Kyyiv, Ukraine, 3 - 06 July 2018, pp.244

  • Publication Type: Conference Paper / Summary Text
  • City: Kyyiv
  • Country: Ukraine
  • Page Numbers: pp.244
  • Kayseri University Affiliated: No


Aim of the study: Lichens have therapeutic effects on various diseases and are often used in

alternative medicine. Lately, certain attention has been paid to lichens as a source of natural

antioxidants. Lichens produce numerous secondary metabolites that attract big attention

because of their antiviral, antimicro bacterial, antitumor, antiallergic, inhibitory activity of

growth of plants and so on. It has been suggested that the extracts of Cladonia species, including

Cladoniaceae family, were used for different medicines in the folk medicine. Lung cancer is the

most common cancer. Therefore, in this study we aimed to investigate the effects of the extracts

of Cladonia fimbriata (L.) Fr. and Cladonia rangiformis Hoffm. on human lung carcinoma (A 549)

with in vitro cytotoxicity assay.

Material and Methods: Within the scope of this study, 70% methanol extracts of Cladonia

species, were prepared. Cytotoxic effects on A 549 cell line were studied with MTT assay.

Results: It has been elucidated that C. rangiformis extract was found to be most cytotoxic

extract with 170.156 μg/mL IC50 value on A 549 cell line. C. fimbriata extract showed 23.40%

inhibition effect on the cell viability even at 1mg/mL concentration. Among the two Cladonia

species studied, C. rangiformis was found promising on A549 cell line. The inhibitory effect of C.

rangiformis extract on lung cancer cell line at different concentrations for 48 and 72 h will be

examined in our subsequent studies.