The 4rd International Symposium on EuroAsian Biodiversity, Kyyiv, Ukrayna, 3 - 06 Temmuz 2018, ss.244
Aim of the study: Lichens have therapeutic effects on various diseases and are often used in
alternative medicine. Lately, certain attention has been paid to lichens as a source of natural
antioxidants. Lichens produce numerous secondary metabolites that attract big attention
because of their antiviral, antimicro bacterial, antitumor, antiallergic, inhibitory activity of
growth of plants and so on. It has been suggested that the extracts of Cladonia species, including
Cladoniaceae family, were used for different medicines in the folk medicine. Lung cancer is the
most common cancer. Therefore, in this study we aimed to investigate the effects of the extracts
of Cladonia fimbriata (L.) Fr. and Cladonia rangiformis Hoffm. on human lung carcinoma (A 549)
with in vitro cytotoxicity assay.
Material and Methods: Within the scope of this study, 70% methanol extracts of Cladonia
species, were prepared. Cytotoxic effects on A 549 cell line were studied with MTT assay.
Results: It has been elucidated that C. rangiformis extract was found to be most cytotoxic
extract with 170.156 μg/mL IC50 value on A 549 cell line. C. fimbriata extract showed 23.40%
inhibition effect on the cell viability even at 1mg/mL concentration. Among the two Cladonia
species studied, C. rangiformis was found promising on A549 cell line. The inhibitory effect of C.
rangiformis extract on lung cancer cell line at different concentrations for 48 and 72 h will be
examined in our subsequent studies.