Usability of some reactive dyes in human blood cells staining

Kuşçulu N., Benli H.

International Journal of Scientific and Technological Research, vol.5, no.2, pp.62-67, 2019 (Peer-Reviewed Journal)


Pomegranate flower plant, popularly known using for the treatment of various diseases, was not investigated as a source of dye for cytological studies using human blood cells. The importance of this study is to appear dyeing result of pomegranate flower extract on human blood cells. The natural dye source was pomegranate flower known as roselle and potassium aluminum sulfate (alum = KAlSO412H2O) was used as mordant or metal salt. Distilled water was used as solvent. Fresh, clean and air-dried flowers were extracted with distilled water at 100 °C for 30 minutes and then filtered. One drop blood from a healthy 20-year woman was spread as a peripheral on to ten plates and dried at nearly 25 °C. These slides were stained by soaking in pomegranate flower extract with/without alum (KAlSO412H2O) at 100 °C for 60 minutes. Slides were washed with distilled water, dried and done microscobic examination. The different blood cells dyed dark orange in alum mordant media at 100 °C. As a result, pomegranate flower has the capacity to use dyeing human blood cells such as eosinophil, basophil and neutrophil.