Evaluation of natural staining on Wistar rat EMI region using different metal salts

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Kuşçulu N.

Journal of Veterinary Health Science, vol.2, no.1, pp.15-18, 2021 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 2 Issue: 1
  • Publication Date: 2021
  • Journal Name: Journal of Veterinary Health Science
  • Journal Indexes: Biotechnology Research Abstracts
  • Page Numbers: pp.15-18
  • Kayseri University Affiliated: Yes


Background: The natural staining of cells has recently become a growing research topic. Roselle (Hibiscus sabdariffa) chemical
structure has two main components that contain more chromophore groups. Objectives: The aim of this study is to dye cells and
elements in two important parts of the Wistar rat uterus with roselle extract containing dense flavonoids and metal salts.
Methods: In this study, dried and clean Hibiscus sabdariffa obtained from the local market was used for histological staining of
uterine layers (EMI region) in Wistar rat tissue. Different cell structures and their different pathological evaluations are made in
the EMI region. In order to more reach different cells, solutions of one percent anhydrous SnCl2 (tin chloride), 1% FeSO4.5H2O
(ferrous sulfate), and 1% KAlSO4.12H2O (alum) metal salts in distilled water were used in this study.
Results: In staining with roselle extracts containing alum and iron sulfate, tissue red-colored endometrial cell staining, and bluepurple
stained inflammatory cells were observed in staining with roselle extract containing 1% SnCl2.
Conclusions: Cytoplasmic and nuclear staining intensity, bluing, and clarity were better when SnCl2 was used as the bluing or
bruising agent. A purple chelates and coordinate covalent bonding between Flavonoids in the roselle structure and the protein
structures in the cell was formed by tin metal in SnCl2 solution. In fact, that there are blue-purple-stained regions in different
shades in the cell structure suggests that flavonoids stain different uterine cells thanks to SnCl2. This situation may help to
evaluate different and complex pathological findings in the uterus