Akademik Veri Yönetim Sistemi
Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, cilt.40, sa.2, ss.304-314, 2024 (Hakemli Dergi)
Ecballium elaterium is a native species of the south of Europe and the Mediterranean catchment, occasionally cultivated for its use as a medicinal plant. In this study was investigated to phytochemicals profiles, cucurbitacin content, antioxidant, DNA cleavage properties and anticancer activity of E. elaterium seed, fruit peel and juice ethanol extracts. Phytochemical components of all extracts were determined by phytochemical analysis methods. Quantitative cucurbitacin content was determined by GC-MS, free radical scavenging activity of the extracts was determined by DPPH method, separation ability on pBR322 DNA was determined by agarose gel electrophoresis and cytotoxic effects were determined by MTT analysis on the HepG2 liver cancer line. Phytochemical and GC-MS analysis results indicate cucurbitacins containing glycoside molecules that can be evaluated as a drug in the structure of the plant. The highest cucurbitacin content of 3.73 % was found in E. elaterium fruit peel. DPPH activity of juice, fruit peel and seed extracts were determined to be 29.39%, 54.12%, and 79.45%, respectively at the highest concentration. All of the extracts had an effect on the superhelix structure of plasmid DNA. There was a decrease in the viability of HepG2 liver cancer cells due to the increase in concentration. After 48 hours of incubation, IC50 values for juice, fruit peel and seed extracts were determined as 309.02 μg/ml, 525.43 μg/ml and 549.54 μg/ml, respectively. It seems that E. elaterium juice, fruit peel and seed extracts have promising potential as future natural anticancer agents in food industry.