Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans


Turan M. G., Kantarci H., Temtek S. D., Cakici O., Cevik S., KAPLAN O. İ.

STAR Protocols, vol.3, no.3, 2022 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 3 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.1016/j.xpro.2022.101498
  • Journal Name: STAR Protocols
  • Journal Indexes: Scopus
  • Keywords: Bioinformatics, Cell Biology, CRISPR, Genetics, Microscopy, Model Organisms, Molecular Biology, Neuroscience
  • Kayseri University Affiliated: No

Abstract

© 2022 The AuthorsHere, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post-microscopy analysis with kymographs. This protocol breaks down all processes into three categories: (1) pre-imaging preparations, (2) preparations for the time of image acquisition, and (3) post-imaging analyses. The protocol can be applied to determine the speed and frequency of moving particles. For complete details on the use and execution of this protocol, please refer to Cevik et al. (2021).