DETERMINATION OF CHEMICAL COMPOSITIONS, ANTIOXIDANT, DNA CLEAVAGE AND BINDING PROPERTIES OF <i>VINCETOXICUM</i> <i>TMOLEUM</i> EXTRACT


Selvi E. K., GÜLER N., GÜVEN S., MAKBUL S.

STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA, vol.2024, no.1, pp.163-174, 2024 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 2024 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.24193/subbchem.2024.1.10
  • Journal Name: STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Central & Eastern European Academic Source (CEEAS)
  • Page Numbers: pp.163-174
  • Kayseri University Affiliated: Yes

Abstract

In the present study, phenolic compounds, antioxidant activities, DNA cleavage and binding effects of Vincetoxicum tmoleum (Vt) collected from west of Anatolia (Manisa city) were investigated. Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2 '-azino-bis (3ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing antioxidant power assay) tests. Besides, the DNA cleavage and binding features of V. tmoleum extract were studied using pBR322 DNA and CT-DNA, respectively. Phenolic compounds of the extract were analyzed by high performance liquid chromatography (HPLC-DAD). As a result, V. tmoleum methanol extract was found to have the high total phenolic and total flavonoid content and antioxidant effect. Strong positive correlations were also found between DPPH and TFC (r = 0.995; p < 0.01), and TPC (r = 0.989;p < 0.01), ABTS (r = 0.994;p < 0.01) and FRAP(r=0,995; p < 0.01). Methanol extract of V. tmoleum had large amounts of p -coumaric acid, ferulic acid and protocatechuic acid. While V. tmoleum samples showed weak DNA cleavage activity, they showed DNA binding activity at 50 mu M concentration, that is, the potential to be an intercalation agent in this concentration.