RAFT-synthesized POEGMA-b-P4VP block copolymers: preparation of nanosized micelles for anticancer drug release


BAYRAM N. N., TOPUZOĞULLARI M., İŞOĞLU İ. A., DİNÇER İŞOĞLU S.

Polymer Bulletin, cilt.79, sa.11, ss.9575-9588, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 79 Sayı: 11
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s00289-021-03964-8
  • Dergi Adı: Polymer Bulletin
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Chemical Abstracts Core, Chimica, Compendex, INSPEC
  • Sayfa Sayıları: ss.9575-9588
  • Anahtar Kelimeler: pH-responsive, Micelle nanocarrier, RAFT, Breast cancer, POLYMERIC MICELLE, DOXORUBICIN, PH, CANCER, DELIVERY, NANOCARRIERS, ACCUMULATION, CHEMOTHERAPY, MOLECULES
  • Kayseri Üniversitesi Adresli: Hayır

Özet

© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.To achieve high stability and biocompatibility in physiological environment, oligoethyleneglycol methacrylate (OEGMA) and 4-vinylpyridine (4VP)-based amphiphilic block copolymers were prepared as micellar carriers to deliver doxorubicin into tumor cells. First, macroinitiator of OEGMA was synthesized by RAFT polymerization at [M]0/[CTA]0/[I]0 ratio of 100/1/0.2 in dimethylformamide (DMF) at 70 °C, in the presence of 4,4′-azobis(4-cyanovaleric acid) (ACVA) as initiator and 4-cyano-4-(thiobenzoylthio)pentanoic acid (CTA) as chain transfer agent, respectively. It was followed by copolymerization with 4-VP at similar conditions. The formation of RAFT-mediated polymers was approved by FTIR, 1H-NMR and GPC. For the preparation of drug-loaded micelles, a dialysis method was applied and hydrophobic doxorubicin, as a model drug, was entrapped into the micelles. Size distributions and morphologies of drug-loaded micelles were investigated by light scattering and scanning electron microscopy, respectively. Critical micelle concentration was estimated as 0.0019 mg/mL by measuring light scattering intensity in different polymer concentrations. Also, drug loading and entrapment efficiencies were calculated as 4.41% and 17.65% by measuring the DOX amount in the micelles, spectrophotometrically. At last, the drug-loaded micelles were applied to SKBR-3 breast cancer cell lines and revealed up to %40 cell inhibition at 48 and 72 h. As a result, these nanosized and biocompatible micelles can be used for the delivery of hydrophobic drugs, and they can also be modified for further targeting and imaging applications toward specific cancer cells. Graphical abstract: [Figure not available: see fulltext.]