Surface-enhanced Raman Scattering (SERS) is an optical detection means that provides utilities over traditional assay detection technologies. In this study, a UV-irradiation was exploited as an ease non-toxic photochemical method for a one-step reduction-deposition of gold nanoparticles (AuNPs) on zinc oxide nanorods supporter (AuNPs@ZnO) patterned on a flexible and porous membrane. Here, we were able to fabricate a self-cleaned and recyclable SERS-active flexible substrate for the quantitative analysis of hazard organic contaminants. The photocatalytic activity of AuNPs@ZnO nanorods was significantly improved under UV- irradiation and after the deposition of AuNPs of similar to 21 nm on the surface of ZnO nanorods of average particle size similar to 60 nm. The morphology, crystallinity, structural phase and optical behaviors of AuNPs@ZnO nanorods modified flexible membrane were characterized using scanning electron microscopy (SEM, SEM-EDX, SEM-Mapping), X-ray powder diffraction (XRD) and SERS analysis. The photocatalytic capabilities of AuNPs@ZnO nanorods modified flexible membrane were exploited to reuse it as SERS substrates for five cycles without losing significant sensitivity through UV-sensitized photocatalytic self-cleaning. As a result, the optimized AuNPs@ZnO; used for SERS analysis of rhodamine 6 G (R6 G) and erythrosine B (EryB), was able to degrade probe-analytes within -10 min and a resultant detection limit of 10(-)(10) and 10(-9)M, respectively. Additionally, the calculated enhancement factor was 4.96 x 10(5) and -1.73 x 10(5) for R6 G and Ery B, respectively. Particularly, this contribution is an attempt to lead the potential of cutting-edge research toward practical food biosensor manufacturing for rapid and sensitive monitoring using without-sample-loss preparation methods.