The textile industry has an important place in global marketing and trade. Both production and consumption of cotton and cotton-based materials were also considerably important. Until now, the essential term for the textile industry can be summarized as product diversity. However, today, in addition, the demand for natural and organic products has been in an increasing trend. In this respect, the use of natural dyes has become popular again. However, the need to use mordants, especially metal salts, is the most important dilemma in terms of natural production. This study was aimed to show the usability of ozone gas with natural dyeing and to introduce an alternative production that avoids use of mordants. It was observed that different ozone gas applications can be used to obtain color diversity from the same natural dye source, which at present is achieved by using different mordants. On the other hand, for the tested dyes, different fastnesses were analyzed in terms of the effect of ozone gas treatment style and different mordants. It was found that in general the tested natural dyes have good fastness values except for light fastnesses. The use of ozone gas only improved the wet rubbing fastness values when ozone gas was applied directly to the wet dyed sample. For other fastnesses, ozone did not show any significant effect.