Inhibition of Plasma Membrane Calcium Pump Influences Intracellular Calcium Signaling Pathways in Breast Cancer

Caner A., ÖNAL M. G.

Cell Biochemistry and Biophysics, vol.80, no.4, pp.747-753, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 80 Issue: 4
  • Publication Date: 2022
  • Doi Number: 10.1007/s12013-022-01090-4
  • Journal Name: Cell Biochemistry and Biophysics
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Page Numbers: pp.747-753
  • Keywords: Breast cancer, Calcium pathway, Orthovanadate, PMCA, CALCINEURIN, CA2+, CALMODULIN, ACTIVATION, EXPRESSION, MIGRATION, HEALTH
  • Kayseri University Affiliated: No


© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.The plasma membrane calcium pump (PMCA) is an important transporter that maintains intracellular calcium concentration ([Ca2+]i). It allows the calcium (Ca2+) from inside the cell to go out of the cell through the plasma membrane. For this, it cooperates with the proteins in the cell. The aim of this study is to demonstrate the effect of PMCA on intracellular calcium signaling in breast cancer cells. In this study, PMCA was inhibited by orthovanadate (OV), and changes in Calmodulin (CaM), Calcineurin (CaN) and cMyc proteins were demonstrated. Intracellular calcium accumulation was measured when PMCA was inhibited in MDA-MB-231 cells. At the same time, it was observed that the cell movement decreased with time. Over time, CaN and CaM were slightly suppressed, and cMyc protein was not expressed. As a result, when PMCA protein is targeted correctly in breast cancer cells, it has an indirect effect on cancer-promoting proteins.