Optimization of ultrasound-assisted aqueous two-phase system (ATPS) of phenolics from apple pulp and peel


İŞÇİMEN E. M., Berktaş S., ÇAM M., HAYTA M.

Preparative Biochemistry and Biotechnology, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2022
  • Doi Number: 10.1080/10826068.2022.2062774
  • Journal Name: Preparative Biochemistry and Biotechnology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Keywords: Apple peel, apple pulp, aqueous two-phase extraction, optimization, ultrasound, ANTIOXIDANT ACTIVITY, FLAVONOID CONTENTS, EXTRACTION, POMACE, ANTHOCYANINS, VALORIZATION, PRODUCTS, MULBERRY, RECOVERY, SOLVENT
  • Kayseri University Affiliated: No

Abstract

© 2022 Taylor & Francis Group, LLC.In this study, extraction of phenolic compounds from apple peel and pulp was optimized using an ultrasound-assisted aqueous two-phase system (UA-ATPS) consisting of acetone-NaH2PO4 and acetone-(NH4)2SO4 with the help of Response Surface Methodology. The effectiveness of UA-ATPS was compared with certain extraction methods including traditional aqueous two-phase system (T-ATPS), ultrasound-assisted acetone extraction (UA-ACE) and traditional acetone extraction (T-ACE). The UA-ATPS gave better results than traditional methods in terms of phenolic and flavonoid contents and antioxidant activity values regardless of the fruit parts used. The status of extraction yield was observed with three major phenolics of apple including chlorogenic acid, rutin and phloridzin. Striking increases in chlorogenic acid and rutin contents in the peel were determined when acetone was replaced with ATPS in the same extraction system with equal extraction time (20 min). The present study has indicated that UA-ATPS is an effective method for phenolic extraction from apple peel and pulp.