UiO-66 metal-organic framework as a double actor in chitosan scaffolds: Antibiotic carrier and osteogenesis promoter

KARAKEÇİLİ A., TOPUZ B., Ersoy F. Ş., Şahin T., Günyakti A., DEMİRTAŞ T. T.

Biomaterials Advances, cilt.136, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 136
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.bioadv.2022.212757
  • Dergi Adı: Biomaterials Advances
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: UiO-66, Wet spinning, Chitosan scaffold, Osteogenic differentiation, Fosfomycin carrier, Osteomyelitis, ANTIBACTERIAL, DIFFERENTIATION, MECHANISMS, MEMBRANES, DELIVERY, CELLS
  • Kayseri Üniversitesi Adresli: Hayır

Özet

© 2022 Elsevier B.V.Metal–organic frameworks (MOFs) have recently emerged as a useful class of nanostructures with well-suited characteristics for drug delivery applications, due to the high surface area and pore size for efficient loading. Despite their use as a nano-carrier for controlled delivery of various types of drugs, the inherent osteo-conductive properties have stolen a great attention as a growing area of investigation. Here, we evaluated the double function of UiO-66 MOF structure as a carrier for fosfomycin antibiotic and also as an osteogenic differentiation promoter when introduced in 3D chitosan scaffolds, for the first time. Our results revealed that the wet-spun chitosan scaffolds containing fosfomycin loaded UiO-66 nanocrystals (CHI/UiO-66/FOS) possessed fiber mesh structure with integrated micro-scale fibers and increased mechanical strength. In vitro antibacterial studies indicated that CHI/UiO-66/FOS scaffolds showed bactericidal activity against Staphylococcus aureus. Moreover, the scaffolds were biocompatible to MC3T3-E1 pre-osteoblasts and significantly up-regulated the expression of osteogenesis-related genes and facilitated the extracellular matrix mineralization, in vitro. Taken together, our results demonstrate UiO-66 MOFs can present double functionality and CHI/UiO-66/FOS scaffolds hold a significant potential to be further explored as an alternative approach in treating infected bone defects like osteomyelitis.