Raman spectroscopy: A novel experimental approach to evaluating cisplatin induced tissue damage


Yay A., Onses M. S. , Sahmetlioglu E., Ceyhan A., Pekdemir S., Önder G. Ö. , ...More

TALANTA, vol.207, 2020 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 207
  • Publication Date: 2020
  • Doi Number: 10.1016/j.talanta.2019.120343
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, Linguistic Bibliography, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Cisplatin, Ovary, Curcumin, Beta-carotene, Raman spectroscopy, ACUTE RENAL INJURY, INDUCED NEPHROTOXICITY, OXIDATIVE STRESS, MOLECULAR-MECHANISMS, CANCER-THERAPY, CURCUMIN, INFLAMMATION, SPECTRA, KIDNEY, LIVER

Abstract

The aim of this work is to clarify the effect of curcumin and beta-carotene on cisplatin-induced tissue damage and to demonstrate the potential of Raman spectroscopy to detect tissue changes consistent with liver and kidney histopathology as a potential diagnostic adjunct. In the study, 56 Wistar albino female rats were used and randomly divided into 7 groups (n:8). Sham group received only sesame oil; Cisplatin group, received a single dose injection of cisplatin; Beta-carotene group, treated with beta-carotene orally; Cisplatin + Beta-carotene group, pretreated with beta-carotene 30 min prior to the cisplatin injection, then received cisplatin; Curcumin group, orally treated with curcumin; Cisplatin + Curcumin group, pretreated with curcumin 30min prior to the cisplatin injection, then received cisplatin. The second application was performed 1 week after the first application. One of the liver and kidney tissues was taken to 10% form for histopathological examinations and the others were taken to -80 degrees C for raman spectroscopy. Received sections were hematoxylin-eosin stained. The avidin-biotin peroxidase method was used for to investigate anti-TNF-alpha and IL1-beta activities. TUNEL method was applied to determine apoptotic cells.