Investigation of chemical activity, SCHIFF base reactions and staining effects of some amino acids by spectrophotometric and theorical methods

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Kuşçulu N., Aslan H. G.

Journal Of The Indian Chemical Society, vol.99, no.2, pp.13-25, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 99 Issue: 2
  • Publication Date: 2022
  • Doi Number: 10.1016/j.jics.2021.100315
  • Journal Name: Journal Of The Indian Chemical Society
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Analytical Abstracts, Chemical Abstracts Core, EMBASE
  • Page Numbers: pp.13-25
  • Kayseri University Affiliated: Yes


In this study, the staining properties of selected amino acids with Brassica oleraceae extract in
alum and alum-free media were investigated. Basic, acidic and neutral amino acids (arginine,
glutamic acid and glycine) were used to investigate the effect of staining. It was determined
that all amino acids were stained in alum media. In the second step, the R group of amino
acids found in the proteins of the cell nucleus was reacted with salicyl aldehyde. This reaction
was successful only with Arginine. The staining properties of the newly formed compound
were also investigated in alum and alum-free environments. Evaluation of the results was
done using FT-IR and 1H NMR methods. All compounds were optimized with the Gaussian
G09 program (DFT/B3LYP/ 6.311 G(d.p) basic set. HOMO, LUMO and HOMO-LUMO gap
values were determined. Chemical reaction capabilities of amino acids were discussed with
the help of HOMO-LUMO gap values.Keywords: Arginine, Glutamic acid, Glycine, Staining
effect, HOMO-LUMO.