© 2022 De Gruyter. All rights reserved.The variability between methods is seen in the existence of hemoglobin (Hb), triglyceride (TG), blood urea nitrogen (BUN) and glucose in the specimens, which can affect the measurement. Therefore, this study aimed to compare boronate affinity chromatography and turbidimetric inhibition immunoassay methods for HbA1c levels in samples containing different levels of Hb, TG, BUN and glucose. In this study, a total of 412 consecutive samples were used for method comparison. The samples were divided into groups according to HbA1c, TG, BUN, Hb, and glucose levels. Clinically significant changes for HbA1c are defined as ≥0.5%. The mean relative difference as % was determined as 1.4% by the Bland-Altman plot of Trinity against Roche. The Passing-Bablok analysis indicated good agreement (r=0.985) between two methods. When compared the group with and without a clinically significant difference, we found differences only between glucose levels (p=0.003). High glucose levels may cause a difference between the methods. Therefore, we suggest that a method's performance and properties should be considered prior to clinical usage.