2nd INTERNATIONAL BAKU CONFERENCE ON SCIENTIFIC RESEARCH, Baku, Azerbaijan, 28 - 30 April 2021, vol.3, no.1, pp.57-62
Natural dyes are obtained from three different sources: plants, animals and minerals. These
dyes have been widely used in the textile, food and pharmaceutical industries for a long time.
However, studies of evaluating the extracts obtained from plants as histological stains have
been conducted recently. In this study, the ability of the with mordant and without mordant
extract obtained from Alkanna tinctoria plant to stain tissues taken from wistar rat kidney was
investigated. For this, 20 g of powdered plant was filtered with Whatman No 1 filter paper
after being kept overnight at room temperature in 200ml 70% Ethyl alcohol. The filtrate was
divided into 4 parts, one of which was left blank, and 3 grams of CuSO4, NiSO4,
KAlSO4.12H2O (alum) mordants were added to the others. After solubility of the mordant, 4
separate unstained preparates were immersed in the solutions after the paraffin was removed.
The solutions were kept in the oven at 60°C for 1 hour. After the preparates were washed with
distilled water, they were dipped in xylene and closed with entellan. 40 × images of tissues
were taken under the light microscope. With the help of extracts from a plant, different colors
were stained in the cytoplasm of epithelial cells in kidney tissue with different mordant
Key words: Alkanna tinctoria, kidney tissue, mordant, natural staining, alkanet