Due to the global competition, the private sector initiatives has become much more important, and in this process, the leadership and management models came to the front especially in health sector. Because, hospitals, which are health enterprises, especially have some basic features that distinguish them from other service or production enterprises. The most important of these basic features is that the service they offer is of decisive importance for human life. It is important in the health sector to investigate the leadership practices in hospitals, the work-family conflict levels of the employees, and the practices of the hospital enterprises. In this context, the aim of the study is to examine the effect of lean leadership behavior on work-family conflict via the mediator role of perceived organizational support. The sample of this research consists of 323 health workers in a public hospital operating in Kayseri region. The results indicate that lean leadership negatively affected work to family conflict, but lean leadership did not significantly affect family to work conflict. It was also concluded that perceived organizational support has a partial mediating role on the relationship between lean leadership and work-family conflict. Keywords: Lean leadership, Work-family conflict, Perceived organizational support