Anti-quorum sensing, antibiofilm, and antibacterial activities of extracts of Centella asiatica L. leaves, and in vitro derived leaves-calli through tissue culture: a potential for biofouling-prevention

Khan M. A., Khan H. M., Ganie I. B., Kumar S., Shahzad A., ÇELİK İ., ...More

Biofouling, vol.38, no.7, pp.715-728, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 7
  • Publication Date: 2022
  • Doi Number: 10.1080/08927014.2022.2117034
  • Journal Name: Biofouling
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Communication Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Metadex, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Page Numbers: pp.715-728
  • Keywords: Centella asiatica, quorum sensing, antibiofilm, phytoconstituents, GC-MS, VIRULENCE FACTOR PRODUCTION, PSEUDOMONAS-AERUGINOSA, MEDICINAL-PLANTS, SOFTWARE NEWS, ANTIOXIDANT, INHIBITION, PYOVERDIN, DISCOVERY, STRATEGY, SYNTHASE
  • Kayseri University Affiliated: No

Abstract

© 2022 Informa UK Limited, trading as Taylor & Francis Group.Extracts of Centella asiatica leaves (LEs), and in-vitro leaf-calli (CEs), were investigated for antibacterial, antibiofilm, and anti-quorum sensing activities. Ethyl acetate extracts from leaves (EALE), leaf-calli (EACE), methanolic extracts from leaves (MELE), and leaf-calli (MECE) showed antibacterial activity; the minimum inhibitory concentrations (MICs) of LEs and CEs ranged from 0.312–2.50 mg ml−1 and 0.625 − 2.50 mg ml−1, respectively. The MICs of EALE and EACE were 2.50 mg ml−1, each, for C. violaceum 12742, and P. aeruginosa PAO1. At sub-MIC levels, EALE and EACE showed anti-quorum sensing (anti-QS) activity, demonstrated by concentration dependent pigment inhibition of C. violaceum 12742. Similarly, EALE and EACE inhibited QS-controlled virulence factors in P. aeruginosa PAO1 (biofilm, pyocyanin, and pyoverdin); again, the inhibition was concentration-dependent. The best effect was at immediate sub-MIC concentration i.e. 1250 µg ml−1. GC-MS analyses revealed the presence of compound 9,12-Octadecadienoic acid, and in silico docking study suggested interactions with QS-receptors CviR', LasI, and LasR proteins for anti-QS activity.