The aim of this study was to investigate antimicrobial and antioxidant activity of Echinops phaeocephalus extracts prepared with different solvents. For antioxidant potential evaluation, extraction of E. phaeocephalus was obtained by three solvents of different polarities including hexane, methanol and chloroform. Antioxidant potential was evaluated by estimating DPPH radical scavenging activity, total phenolic activity and total flavonoid activity assay Antimicrobial activity was determined by minimal inhibitory concentration (MIC) and disc diffusion tests. Efficiency of different solvents was compared for the yield of antioxidant extracts from samples. The study results indicated that, E. phaeocephalus could be an important natural antioxidant. The evaluations of solvents for effect of extraction resulted that methanol was more effective than other solvents. Echinops phaeocephalus derived extracts were observed to have varies effects on microorganisms. The results of the antioxidant activity assay indicated that the highest phenolic content was in the extract of hexane, while the highest flavonoid content was in methanol extract. Especially methanol and hexane extracts showed high efficiency according to DPPH radical scavenging activity results. Our results concluded that E. phaeocephalus sustains an important antioxidant and antimicrobial activity.