© 2022, Iranian Chemical Society.A fast and straight-forward magnetic dispersive solid-phase microextraction procedure was developed for vanadium(V) determination in some tea samples. For this reason, a new nanocomposite reacted mercaptosuccinic acid (MSA) with Fe3O4/GO through ethylenediamine (EDA) magnetic graphene oxide (Fe3O4/GO/MSA/EDA) was first synthesized and characterized. The effective factors that influence the efficiency of a developed method such as pH, the amount of Fe3O4/GO/MSA/EDA nanocomposite, the concentration of acid solution for desorption cycles, and the amount of sodium dodecyl sulfate and foreign ion effect, etc. were investigated. These magnetic nanocomposite carrying vanadium(V) were easily separated from tea samples simply via external magnetic fields; no centrifugation was necessary. The technique was successively applied to determine vanadium(V) in some tea samples. The method's accuracy was checked via analysis of certified reference materials and spiked tests. The detection limit and quantification limit were found to be 3.74 and 12.34 µg L−1, respectively. The precision of results, expressed as relative standard deviation, was always lower than 1.87%.