Vitamin B12 reduces the negative effects of nicotine on fetal bone development in the rats

Creative Commons License

Payas A., Ekinci Y., Gürbüz K., Batın S., Ertekin T., Doğan K., ...More

Joint Diseases and Related Surgery, vol.33, no.1, pp.216-224, 2022 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.52312/jdrs.2022.529
  • Journal Name: Joint Diseases and Related Surgery
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.216-224
  • Keywords: Enterobacter cloacae, open knee wound, prosthetic joint infection, traumatic knee injury, HIP
  • Kayseri University Affiliated: No


© 2022. All right reserved by the Turkish Joint Diseases FoundationObjectives: The aim of this experimental rat study was to investigate the effectiveness of vitamin B12 (Vit-B12) against the teratogenic effects of nicotine use during pregnancy in the fetus. Materials and methods: Eighteen Wistar-Albino pregnant rats were divided into six groups (n=3, each) as Group 1 (control), Group 2 (Vit-B12), Group 3 (nicotine 3 mg/kg/day), Group 4 (nicotine 3 mg/kg/day + Vit-B12), Group 5 (nicotine 6 mg/kg/day) and Group 6 (nicotine 6 mg/kg/day + Vit-B12). Nicotine was administered subcutaneously and Vit-B12 was administered intraperitoneally for 20 days. The skeletal systems of the embryos were stained using the double-staining method. The total bone length (TBL), the length of the ossified part, and the ossification rate were calculated using the ImageJ program. In addition, the weight and crown-rump length (CRL) of the embryos were measured and recorded. Results: Clinical shock effect was observed in all rats administered nicotine. A significant decrease in weight, CRL, and TBL was measured in Group 3 (p=0.0081) and, particularly in Group 5 (p=0.026), which were given only nicotine, compared to other groups. In the morphometric measurements of Group 4 and Group 6 to which Vit-B12 was added, values close to the control group were measured (p=0.173). The data of Group 6 were statistically significantly larger than Group 5 (p=0.007), while it was statistically smaller than Group 1 (p=0.0038). Conclusion: Nicotine use negatively affects the morphological development of the embryo. On the other hand, Vit-B12 provides benefits in reducing these negative effects.