© 2022 Taylor & Francis Group, LLC.This study aimed to evaluate the hyaluronidase and gelatinase inhibitory activities and toxicity potentials of Persea americana Mill. extract and its synthesized hybrid nanoflowers. In the first part of the study, characterization of the nanoflower structures was carried out and then, enzyme inhibitory activities of the methanol extract and hybrid nanoflowers were investigated. It was determined that the enzyme inhibitory activity of both nanoflowers was higher than the methanol extract. Zinc nanoflower was more effective in hyaluronidase enzyme inhibition, while copper nanoflower showed higher inhibitory activity on gelatinase enzyme. In the second part of the study, toxicological profiles of these compounds were investigated. Toxicological evaluations demonstrated that zinc nanoflower may be a safer therapeutic alternative than the copper nanoflower, especially at high concentrations. All these data appear to contribute to the development of effective new generation preparations including nanoflowers for skin problems using Persea americana leaves extract and its nanostructures.