international Journal of pharmacy & pharmaceutical research, vol.11, no.3, pp.271-276, 2018 (Peer-Reviewed Journal)
The staining of anemic human blood cells by red cabbage
(Brassica oleracea) extract has been investigated. Potassium
aluminum sulfate (alum, KAlSO4∙12H2O), FeSO4, CuSO4∙5H2O
or K2Cr2O7 were used as mordants. Red cabbage leaves were
extracted with distilled water at 100°C for 30 min and the liquid
portion was separated from the precipitate by filtration. A twodrop
blood sample was obtained from a 30-year-old male
anemia patient. The sample was spread as a peripheral smear on
ten glass slides and allowed to dry at room temperature. The
slides were immersed into beakers, which contained red
cabbage extract. Every extract included, respectively, alum,
FeSO4, CuSO4∙5H2O or K2Cr2O7. However, mordant was not
added to one beaker. The beakers were kept in the oven at 50°
C for 1 hrs. The staining procedure was repeated at100°C for
one hour. The slides were then washed with distilled water, airdried
and viewed at 1000 magnification. As a result, we found
that blood cells stained at high temperature and with mordant.
Blood cells were not effectively stained without alum and at
50°C. However, different blood cells were stained with
different mordants at 100°C for one hour in different colors.
Brassica oleracea has the potential for use as a stain for studies
of anemic human blood cells.