THE PROTECTIVE EFFECTS OF DILL (Anethum graveolens L.) IN PARACETAMOL-INDUCED ACUTE TOXICATION IN MICE


Korkmaz S. , Koç F.

International Journal of Agricultural and Natural Sciences, cilt.14, sa.1, ss.47-60, 2021 (Diğer Kurumların Hakemli Dergileri)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Konu: 1
  • Basım Tarihi: 2021
  • Dergi Adı: International Journal of Agricultural and Natural Sciences
  • Sayfa Sayıları: ss.47-60

Özet

The purpose of this study
was
to determine whether the protective effect of the AG (
Anethum
graveolens
) extract against experimental liver acute toxication formed by PT in mice. For this purpose, 2
months old, 20
-
25 g weight Balb
-
c male mice of 50 divided
into 5 groups which each contain 10.
Applications were made by oral gavage. Group I is designated as the control group and isotonic NaCl given
for 7 days, Group II of animals 400 mg/kg a single dose PT applicated orally, Group III is PT+AG1 (100
mg/kg AG g
iven for 7 days and 1 hours after last extract application 400 mg/kg PT given, Group IV is
PT+AG2 (200 mg/kg AG+PT 400 mg/kg), Group V. AG2 (200 mg/kg AG were given orally for 7 days.
About 5 hours after these applications the blood was taken from heart un
der ketamin and xylazine
anesthesia; liver and kidney tissue were taken after cervical dislocation. Assessments were performed serum
cytokine levels (interleukin
-
1ß, interleukin
-
6, interleukin
-
10 and tumor necrosis factor
-
α), some
biochemical enzymes (ALT,
AST), lipid peroxidation levels in tissues (MDA, SOD, GSH) with liver and
kidney histopathological examinations. In our study, it is determined that AST, ALT, cytokine and tissue
MDA levels increased, SOD and GSH levels decreased in PT group. On the other
hand, when we compare
the groups received two different doses (100 and 200 mg/kg) of extract with only PT applied group, AG
application significantly decreased IL
-
1β, IL
-
10, IL
-
6, TNF
-
α, ALT and AST levels in serum. It is
determined that MDA level decreas
ed, and GSH and SOD levels increased depending on dose.
Histopathological findings are consistent with biochemical findings. As a result, AG applications can
prevent organ damage via affected cytokine answer and oxidative stress in PT
-
induced acute liver a
nd
kidney toxications in mice