© 2022 American Association of OrthodontistsIntroduction: The purpose of this study was to assess the effects of orthodontically aimed low-magnitude, high-frequency mechanical vibration (OLMHFMV) on intact calvarial bone, specifically the parietal and temporal, and cranial sutures, including the sagittal and parietotemporal, of rats in differing stages of growth and development. Methods: Forty Wistar rats were divided into 4 groups: 2 control groups and 2 OLMHFMV groups. Subsequently, 0.3 cN of force with a frequency of 30 Hz was applied as OLMHFMV on the temporomandibular joint region in the rats in the OLMHFMV-1 group, with the protocol of 20 min/d for 5 d/wk for 1 month, whereas the rats in the OLMHFMV-2 group received mechanical stimuli for 2 months with the same protocol. Morphometric and structural analyses, including suture width, cranial width and height, bone mineral density, bone volume/tissue volume, trabecular number, trabecular separation, and trabecular thickness analyses, were carried out using micro-computed tomography. Results: The width of the parietotemporal and sagittal sutures and the cranial height and width increased significantly by OLMHFMV (P <0.021). The structural analysis revealed that trabecular number and trabecular separation increased, whereas trabecular thickness decreased in the OLMHFMV groups compared with the control groups (P <0.048). Bone volume/tissue volume remained unchanged despite reducing the bone mineral density of the OLMHFMV groups. Conclusions: OLMHFMV had a potential for modulating sutural and cranial growth in adolescent rats. OLMHFMV increased the structural quality of the temporal and parietal bones. These effects may have clinical implications as a treatment option for patients suffering from craniofacial anomalies such as craniosynostosis or a supportive approach for dentofacial orthodontic treatments.