Synthetic fibers are in increasing demand because of the increasing world population. As a result of this, studies on synthetic fibers have become popular. These studies are especially focused on the modification of synthetic fibers to gain natural fiber like properties and to improve the dyeability of the fibers. The aim of this paper was to investigate the modification opportunities of polyamide fibers with pepsin and trypsin enzymes and the dyeability with natural dye sources. At present there are many different methods to modify the polyamide fibers, however enzymatic modification was examined in this study. As well known, enzymatic processes are generally environmentally friendly so it was planned to complete the finishing of polyamide fabrics by dyeing them via natural dye sources. In the light of these arguments different protease enzymes were used for the modification of polyamide samples in this study.. The modified fabrics were dyed with different natural dyes to investigate the change in dyeability. For this aim color efficiencies and CIE L*a*b* color values were analyzed. Moreover, the surface modification and structural changes were also investigated by the help of SEM and FTIR. It was found that the tested enzymes did not significantly affect the dyeability of the polyamide samples with selected natural dye sources however these natural dye sources was found as an alternative for the coloration of polyamide samples and except light fastnesses, the fastnesses of the dyed fabrics were found sufficient.