Morphological Anatomical and Molecular Distinction of Systematically Confused Cladonia conista and Cladonia humilis


Kocakaya Z. , Kocakaya M., Barak M. Ü. , Aygül E. Ö.

2nd International Eurasian Mycology Congress (EMC’2019), Konya, Turkey, 6 - 09 September 2019, pp.154

  • Publication Type: Conference Paper / Summary Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.154

Abstract

Cladonia genus is a very polymorphic genus. It is a genus with more than 400 species on worldwide. The some

Cladonia species are known to be morphologically variable species. In recent years, the phylogeny of the genus Cladonia

is under investigation. These studies are mostly in the form of comparison of groups or species. The genus Cladonia is

divided into different infrageneric groups studied by many researchers in terms of phylogeny. The high morphological

variability of Cladonia species is caused by factors such as light, temperature or humidity. C. conista and C. humilis in the

Cladonia chlorophaea group are systematically confused with each other. Samples were collected from different regions

of our country. The samples were examined under microscope and morphological and anatomical features were determined.

The characters used to distinguish the species in the group include: the presence of sorediate in the podetium, farinose to

granular soredia, corticate granules, corticate podetial stalk and the presence of bourgeanic acid or atranorin in the thallus.

In this group, the secondary metabolites are fumarprotocetraric acid, bourgeanic acid and atranorin . DNA isolations of the

samples were performed. A phylogenetic tree was constructed with ITS sequences. Although the species in the Cladonia

chlorophaea group are similar, there are some distinctive differences. Morphological, anatomical and ecological differences

of C. conista and C. humilis species were determined. Molecular differences are shown in the phylogenetic tree created

with ITS sequences.