The use of novobiocin and cefixime-tellurite in broth, and of cefixime-tellurite, salicin, rhamnose and MUG in agar medium for the detection of Escherichia coli O157:H7 in ground beef
Turkish Journal of Veterinary and Animal Sciences, cilt.28, sa.4, ss.753-759, 2004 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 4
- Basım Tarihi: 2004
- Dergi Adı: Turkish Journal of Veterinary and Animal Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.753-759
- Kayseri Üniversitesi Adresli: Hayır
Özet
We aimed to improve the selectivity of lauryl tryptose (LST) broth and HC (hemorrhagic colitis Escherichia coli strains) agar for the detection of E. coli O157:H7 in frozen ground beef samples. The selectivity of LST broth was superior to that of LST containing novobiocin (20 mg/l) (LST + n), but LST containing novobiocin, cefixime (0.05 mg/l) and tellurite (2.5 mg/l) (LST + nct) was found to be unsuitable as an enrichment medium. The sorbitol positive strains were significantly more resistant to novobiocin than were the sorbitol negative strains (P < 0.05). As a result, the enrichment of ground beef samples in a selective broth without antibiotics such as LST, and then spread plating a subsequent amount from this original enrichment culture and the first three 10-fold serial dilutions onto a rhamnose and sorbitol based selective medium such as cefixime-tellurite HC medium (CTR-HC) was found to be recommendable as a routine cultural method. Transferring the metallic sheen property of eosin methylene blue (EMB) agar to CTR-HC agar can represent an alternative to the available media, and this will shorten the analysis time by 1 day. Use of salicine or 4-methylumbelliferyl-b-D glucuronide appears unnecessary in routine cultural techniques.