Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole


Sahmetlioglu E. , YURUK H., TOPPARE L., Cianga I., YAGCI Y.

REACTIVE & FUNCTIONAL POLYMERS, vol.66, no.3, pp.365-371, 2006 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 66 Issue: 3
  • Publication Date: 2006
  • Doi Number: 10.1016/j.reactfunctpolym.2005.08.009
  • Title of Journal : REACTIVE & FUNCTIONAL POLYMERS
  • Page Numbers: pp.365-371

Abstract

In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was examined. The optimum temperature, operational and storage stabilities of immobilized enzymes were determined. PVATh/PPy copolymer was found to exhibit significantly enhanced properties compared to pristine polypyrrole. (c) 2005 Elsevier B.V. All rights reserved.