Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole
REACTIVE & FUNCTIONAL POLYMERS, cilt.66, sa.3, ss.365-371, 2006 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 66 Sayı: 3
- Basım Tarihi: 2006
- Doi Numarası: 10.1016/j.reactfunctpolym.2005.08.009
- Dergi Adı: REACTIVE & FUNCTIONAL POLYMERS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.365-371
- Anahtar Kelimeler: immobilization, invertase, glucose oxidase, poly(vinyl alcohol), electropolymerization
- Kayseri Üniversitesi Adresli: Hayır
Özet
In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was examined. The optimum temperature, operational and storage stabilities of immobilized enzymes were determined. PVATh/PPy copolymer was found to exhibit significantly enhanced properties compared to pristine polypyrrole. (c) 2005 Elsevier B.V. All rights reserved.