© 2021, The Author(s), under exclusive licence to Springer Nature B.V.The current study aimed at estimating the direct (condemned offal) and indirect (meat, milk, and fecundity losses) production losses due to cystic echinococcosis (CE) in water buffaloes in Turkey. A spreadsheet loss model was constructed, and the mean prevalence ratio of CE was accepted as 3.8% in male and 21.7% in female buffaloes. The annual financial losses were estimated with official and previously published data under expected (mean value), optimistic (lowered by 10%), and pessimistic (increased by 10%) scenarios with the prices current in 2021. The production losses in an infected male and female water buffalo were estimated at $54.5 ($49.1–59.9) and $89.9 ($80.9–98.9), respectively. Due to CE, the nationwide annual total production losses were estimated at $1.5 million ($1.3–1.6) for water buffaloes in Turkey. In conclusion, farmers, policymakers, and the public need to be informed about CE’s risks and financial impact. Control/eradication programs should be included in government policies at the national level after a cost/benefit analysis.